Multiple Choice
Which of the following events causes recombination between linked genes to occur in organic chemistry reactions?
3. Acids and Bases
Organic Chemistry Reactions
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In the process of cloning a gene into a plasmid vector, which step requires the use of restriction enzymes?
A
Amplifying the gene using PCR
B
Transforming the plasmid into bacterial cells
C
Ligating the gene into the plasmid
D
Cutting the plasmid and gene at specific sites
Verified step by step guidance1
Understand that restriction enzymes are proteins that cut DNA at specific sequences, known as restriction sites.
Identify the role of restriction enzymes in the cloning process: they are used to cut both the plasmid vector and the gene of interest at specific sites to create compatible ends.
Recognize that amplifying the gene using PCR does not involve restriction enzymes; PCR is used to make multiple copies of the gene.
Note that transforming the plasmid into bacterial cells is a separate step where the plasmid is introduced into bacteria, not involving restriction enzymes.
Conclude that the step requiring restriction enzymes is the cutting of the plasmid and the gene at specific sites to prepare them for ligation.
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