Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

7. Enzyme Inhibition and Regulation

Sequential (KNF) Model

Biochemistry

7. Enzyme Inhibition and Regulation

Sequential (KNF) Model

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Multiple Choice

The Sequential model for allosteric enzyme behavior:

Cannot account for the reactions that display negative cooperativity.

Postulates binding of substrates & inhibitors by the induced-fit model.

Requires that the conformation of all subunits change simultaneously.

Is conceptually and mathematically simpler than the concerted model.

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Verified step by step guidance

Begin by understanding the concept of allosteric enzymes, which are enzymes that change their conformation upon binding of an effector, resulting in an altered activity at the active site.

Recognize that the Sequential model, also known as the Koshland-Némethy-Filmer (KNF) model, describes how substrate binding induces a conformational change in one subunit, which then affects neighboring subunits.

Note that the Sequential model postulates binding of substrates and inhibitors by the induced-fit model, meaning that the binding of a substrate induces a change in the enzyme's shape to accommodate the substrate.

Understand that the Sequential model does not require all subunits to change conformation simultaneously, unlike the concerted model, which assumes a simultaneous change in all subunits.

Acknowledge that the Sequential model is conceptually and mathematically simpler than the concerted model, as it allows for intermediate states and does not require all subunits to be in the same state at any given time.