Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

4. Protein Structure

Denaturation

Biochemistry

4. Protein Structure

Denaturation

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Multiple Choice

Which of the following is least likely to result in protein denaturation?

Changing pH

Changing the [salt].

Disruption of weak interactions by boiling the protein solution.

Exposure to chaotropic agents & detergents (such as urea & SDS).

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Verified step by step guidance

Understand the concept of protein denaturation: Protein denaturation involves the unfolding and loss of the protein's native structure, which is often caused by the disruption of non-covalent interactions such as hydrogen bonds, ionic bonds, and hydrophobic interactions.

Consider the effect of changing pH: Altering the pH can lead to the protonation or deprotonation of amino acid side chains, disrupting ionic bonds and hydrogen bonds, which can result in denaturation.

Evaluate the impact of changing salt concentration: Moderate changes in salt concentration can stabilize proteins by shielding charges on the protein surface, but extreme changes can lead to denaturation by disrupting ionic interactions.

Analyze the effect of boiling: High temperatures increase kinetic energy, leading to the disruption of hydrogen bonds and hydrophobic interactions, causing denaturation.

Assess the role of chaotropic agents and detergents: These substances disrupt the hydrophobic core of proteins and interfere with hydrogen bonding, leading to denaturation.