Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

5. Protein Techniques

Column Chromatography

Biochemistry

5. Protein Techniques

Column Chromatography

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Multiple Choice

Which of the following techniques directly allows for effective separation/purification of a target protein?

Dialysis.

Salting out.

Chromatography.

Homogenization.

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Verified step by step guidance

Understand the purpose of each technique: Dialysis is used to remove small molecules from a solution, salting out is used to precipitate proteins by altering solubility, and homogenization is used to break cells open. Chromatography, however, is specifically designed for separating and purifying proteins based on different properties.

Recognize that chromatography is a versatile technique that can separate proteins based on size, charge, hydrophobicity, or specific binding affinity, depending on the type of chromatography used (e.g., size-exclusion, ion-exchange, affinity chromatography).

Consider the principle of chromatography: it involves passing a mixture dissolved in a 'mobile phase' through a 'stationary phase'. Different components of the mixture travel at different speeds, leading to separation.

Identify the type of chromatography that would be most effective for your target protein. For example, if the protein has a unique binding site, affinity chromatography might be the best choice.

Plan the chromatography process: prepare the column with the appropriate stationary phase, load your protein mixture, and then elute the proteins while collecting fractions. Analyze these fractions to identify the one containing your target protein.