Multiple Choice
Which of the following techniques directly allows for effective separation/purification of a target protein?
5. Protein Techniques
Column Chromatography
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Which of the following is true regarding the stationary phase?
A
The stationary phase flows over the mobile phase to separate proteins.
B
The protein sample is placed on the stationary phase & the mobile phase flows over it.
C
The stationary phase interacts with the mobile phase as they both move through the column.
D
Proteins with the strongest affinity to the stationary phase are eluted first.
Verified step by step guidance1
Understand the concept of chromatography: Chromatography is a technique used to separate components in a mixture based on their interactions with a stationary phase and a mobile phase.
Define the stationary phase: The stationary phase is the solid or liquid phase that remains fixed in place within the chromatography column. It interacts with the components of the mixture.
Define the mobile phase: The mobile phase is the liquid or gas that flows over or through the stationary phase, carrying the components of the mixture with it.
Analyze the interaction: Proteins or other molecules in the sample will interact differently with the stationary phase based on their properties, such as size, charge, or affinity.
Determine the elution order: Proteins with stronger affinity to the stationary phase will be retained longer and eluted later, while those with weaker affinity will elute first.
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Column Chromatography practice set
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