Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

8. Protein Function

Introduction to Protein-Ligand Interactions

Biochemistry

8. Protein Function

Introduction to Protein-Ligand Interactions

Previous problemNext problem

Struggling with Biochemistry?

Join thousands of students who trust us to help them ace their exams!Watch the first video

Multiple Choice

Calculate the dissociation rate constant (k_d) at equilibrium if [P] = 20 mM, [L] = 10 mM, [PL] = 5 mM, and the association rate constant (k_a) = 100 mM^-1s^-1.

400.

4,000 s^-1.

4,000 mM^-1s^-1.

Previous problemNext problem

Verified step by step guidance

Understand the relationship between the association rate constant (ka), dissociation rate constant (kd), and the equilibrium concentrations of the protein (P), ligand (L), and the protein-ligand complex (PL). The equilibrium constant (K) is given by the ratio of kd to ka.

The equilibrium constant (K) can be expressed in terms of the concentrations: \( K = \frac{[P][L]}{[PL]} \). This equation represents the ratio of the concentrations of the free protein and ligand to the concentration of the protein-ligand complex at equilibrium.

Rearrange the equation to solve for the dissociation rate constant (kd): \( kd = ka \times K \). This step involves substituting the expression for K into the equation for kd.

Substitute the given values into the equation: \( K = \frac{20 \text{ mM} \times 10 \text{ mM}}{5 \text{ mM}} \). Calculate the value of K using the provided concentrations.

Finally, calculate kd using the formula \( kd = 100 \text{ mM}^{-1}\text{s}^{-1} \times K \). This step involves multiplying the association rate constant (ka) by the calculated equilibrium constant (K) to find the dissociation rate constant (kd).