Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

7. Enzyme Inhibition and Regulation

Noncompetitive Inhibition

Biochemistry

7. Enzyme Inhibition and Regulation

Noncompetitive Inhibition

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Multiple Choice

How would you expect the line on a Lineweaver-Burk plot to change if the enzyme was treated with a noncompetitive inhibitor?

The y-intercept would move up (away from the origin).

The x-intercept would move left (away from the origin).

The y-intercept would move down (toward the origin).

The x-intercept would move right (toward the origin).

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Verified step by step guidance

Understand the Lineweaver-Burk plot: It is a double reciprocal plot of the Michaelis-Menten equation, where 1/V (reaction velocity) is plotted against 1/[S] (substrate concentration). The y-intercept represents 1/Vmax and the x-intercept represents -1/Km.

Recognize the effect of a noncompetitive inhibitor: Noncompetitive inhibitors bind to an enzyme at a site other than the active site, reducing the overall number of active enzyme molecules but not affecting the binding of substrate to enzyme.

Determine the impact on Vmax: Noncompetitive inhibition decreases the maximum reaction velocity (Vmax) because it reduces the number of active enzyme molecules. This means the y-intercept (1/Vmax) will increase, moving up on the plot.

Assess the impact on Km: Since noncompetitive inhibitors do not affect the binding of substrate to enzyme, the Km remains unchanged. Therefore, the x-intercept (-1/Km) does not move.

Summarize the changes on the plot: With a noncompetitive inhibitor, the y-intercept moves up (away from the origin) due to a decrease in Vmax, while the x-intercept remains unchanged because Km is unaffected.