Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

6. Enzymes and Enzyme Kinetics

Vmax Enzyme

Biochemistry

6. Enzymes and Enzyme Kinetics

Vmax Enzyme

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Multiple Choice

In a Michaelis-Menten kinetics plot (V0 vs. [S]), what is the reason that the curve reaches a plateau and V0 cannot increase any further upon adding more substrate?

The enzyme becomes locked in an inactive conformation.

Enzymes match rate of catalysis & rate of ES formation.

The active site of all the enzymes are saturated with substrate.

There is an inhibitor present.

Vmax can only be attained by some enzymes.

Enzyme is locked in an inactive conformation.

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Verified step by step guidance

Understand the concept of Michaelis-Menten kinetics: This model describes the rate of enzymatic reactions by relating reaction rate (V0) to substrate concentration ([S]). The key parameters are Vmax (maximum rate) and Km (Michaelis constant).

Recognize the significance of Vmax: Vmax represents the maximum rate of the reaction when the enzyme is saturated with substrate. It is the plateau observed in the Michaelis-Menten plot.

Identify the reason for the plateau: The plateau occurs because all active sites of the enzyme molecules are occupied by substrate, meaning the enzyme is working at its maximum capacity.

Consider the role of substrate concentration: As substrate concentration increases, the reaction rate increases until all enzyme active sites are occupied. Beyond this point, adding more substrate does not increase the rate further.

Clarify the concept of enzyme saturation: Enzyme saturation means that every enzyme molecule is bound to a substrate molecule, and the reaction rate is at its maximum (Vmax). This is why the curve reaches a plateau in the plot.