Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

6. Enzymes and Enzyme Kinetics

Calculating Vmax

Biochemistry

6. Enzymes and Enzyme Kinetics

Calculating Vmax

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Multiple Choice

Triose phosphate isomerase catalyzes the conversion of dihydroxyacetone phosphate (DHAP) to glyceraldehyde-3-phosphate (G3P) during glycolysis; however, this is a reversible reaction. The K_m of the enzyme for G3P is 1.8 x 10 ^-5 M. When [G3P] = 30 μM, the initial rate of the reaction (V₀) = 82.5 μmolemL^-1sec^-1. Calculate the V_max.

0.493 M s^-1.

1.201 M s^-1.

0.067 M s^-1.

0.132 M s^-1.

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Verified step by step guidance

Understand that the problem involves the enzyme kinetics of triose phosphate isomerase, which catalyzes the interconversion between DHAP and G3P. The reaction is reversible, and we are given the Km for G3P and the initial rate V0 at a specific concentration of G3P.

Recall the Michaelis-Menten equation: V0 = (Vmax * [S]) / (Km + [S]), where V0 is the initial rate, Vmax is the maximum rate, [S] is the substrate concentration, and Km is the Michaelis constant.

Substitute the given values into the Michaelis-Menten equation: V0 = 82.5 μmole*mL-1*sec-1, [S] = 30 μM (which is 30 x 10^-6 M), and Km = 1.8 x 10^-5 M.

Rearrange the equation to solve for Vmax: Vmax = V0 * (Km + [S]) / [S].

Convert the units of V0 from μmole*mL-1*sec-1 to M s-1 by considering the conversion factor (1 M = 1000 μmole/mL), and then calculate Vmax using the rearranged equation.