Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

5. Protein Techniques

SDS-PAGE

Biochemistry

5. Protein Techniques

SDS-PAGE

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Multiple Choice

Which of the following statements are true regarding the treatment of proteins with SDS?
i) Only proteins with native net charges acquire an overall net negative charge.
ii) Proteins denature due to a disruption of the hydrophobic interactions stabilizing the core of their structures.
iii) All protein subunits can be separated via SDS-PAGE.

i, ii, iii.

i, ii.

ii, iii.

i, iii.

ii.

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Verified step by step guidance

Understand the role of SDS (sodium dodecyl sulfate) in protein analysis: SDS is an anionic detergent that binds to proteins, giving them a uniform negative charge and denaturing them by disrupting non-covalent bonds.

Evaluate statement i: SDS imparts a negative charge to all proteins, not just those with native net charges. Therefore, this statement is false.

Evaluate statement ii: SDS denatures proteins by disrupting hydrophobic interactions, which are crucial for maintaining the protein's tertiary structure. This statement is true.

Evaluate statement iii: SDS-PAGE separates proteins based on size, not charge or subunit composition. While SDS can separate subunits of a protein complex, it does not guarantee separation of all subunits if they are covalently linked. This statement is partially true but not universally applicable.

Conclude which statements are true: Based on the evaluations, only statement ii is consistently true regarding the treatment of proteins with SDS.