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Multiple Choice
In the following HPLC chromatogram, which amino acid was the third substance eluted from the column?
A
Aspartic acid.
B
Leucine.
C
Phenylalanine.
D
Glutamic Acid.
Verified step by step guidance
1
Examine the HPLC chromatogram image provided, focusing on the peaks and their corresponding labels.
Identify the sequence of peaks from left to right, as this represents the order in which substances are eluted from the column.
Locate the third peak in the sequence and note the label associated with this peak.
Understand that each label corresponds to a specific amino acid, which is eluted at a particular time based on its interaction with the column material.
Determine the amino acid associated with the third peak by matching the label to the list of amino acids provided in the problem statement.