If Mb's K d = 2.5 M and the [O 2 ] = 7.5 M, what % saturated will Mb be?

So this practice problem asks, if myoglobin's kd is equal to 2.5 molar and the concentration of oxygen gas is equal to 7.5 molar, what percentage saturated will myoglobin be? And we've got these 4 potential answer options down below. Now, in order to determine the percent saturation, we're going to need to calculate the fractional saturation. And so notice down below in this box I have the fractional saturation that relates, the fractional saturation to the k d, which is what we're given. And so notice that we are given both the concentration of oxygen at 7.5 molar, as well as the kd as 7.5 molar as well as the KD as a 2.5 molar. So all we need to do is plug in these values. And so when we do that, what we get is, the concentration of oxygen is 7.5 molar on top here. And this is going to be over 7.5 Molar, plus the k d, which again is given to us as 2.5 here, so I'll put this here in red. And this, is something that we can just type into our calculator. So if we take 7.5 molar and divide it by 7.5 molar plus 2.5 molar, what we'll get is an answer of 0, 0.75. Now, 0.7 5 is actually the fractional saturation theta. However, it's asking us about a percentage and so, in order to convert this, theta of 0.75 into a percentage, we need to further multiply this by 100%. And so when we do that, what we get is 70 5%. And so, it's this percentage at the end here, 75%. That is our answer. And so, under these conditions stated, here, Myoglobin is going to be 75% saturated with oxygen and so we can indicate that option b here is the correct answer and that concludes this practice. So, I'll see you guys in our next video.

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8. Protein Function

Myoglobin vs. Hemoglobin

Biochemistry

8. Protein Function

Myoglobin vs. Hemoglobin

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Multiple Choice

If Mb's K_d = 2.5 M and the [O₂] = 7.5 M, what % saturated will Mb be?

12%

75%

0.75%

64%

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Verified step by step guidance

Understand the concept of saturation: Myoglobin (Mb) saturation refers to the fraction of Mb molecules that have bound oxygen (O2). The percentage saturation can be calculated using the formula for fractional saturation.

Use the formula for fractional saturation: The fractional saturation (Y) can be calculated using the equation:

Y = \frac{[O 2]}{K d + [O 2]}

, where [O2] is the concentration of oxygen and Kd is the dissociation constant.

Substitute the given values into the formula: Plug in the values for [O2] = 7.5 M and Kd = 2.5 M into the equation:

Y = \frac{7.5}{2.5 + 7.5}

Calculate the fractional saturation: Perform the arithmetic to find the value of Y, which represents the fraction of Mb that is saturated with oxygen.

Convert fractional saturation to percentage: Multiply the fractional saturation (Y) by 100 to convert it into a percentage, which will give you the % saturation of Mb.