Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

5. Protein Techniques

Native Gel Electrophoresis

Biochemistry

5. Protein Techniques

Native Gel Electrophoresis

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Multiple Choice

Which option below best describes the native gel electrophoresis migration for Proteins A, B, C & D (assuming equal mass & shape) considering that the buffer solution has a pH = 6.4.
Protein A pI = 5.2, Protein B pI = 6.4, Protein C pI = 7.0, Protein D pI = 9.2

A & B will migrate to the negative pole while C & D migrate to the positive pole.

A will migrate to the positive pole, B will not migrate, while C & D migrate to the negative pole.

A & B will migrate to the positive pole while C & D migrate to the negative pole.

A will migrate to the negative pole, B will not migrate, while C & D migrate to the positive pole.

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Verified step by step guidance

Understand the concept of isoelectric point (pI): The isoelectric point is the pH at which a protein carries no net charge. At a pH below its pI, a protein will carry a positive charge, and at a pH above its pI, it will carry a negative charge.

Determine the charge of each protein at pH 6.4: Compare the pH of the buffer (6.4) with the pI of each protein to determine their net charge.

Analyze Protein A: Since the pI of Protein A is 5.2, which is less than 6.4, Protein A will have a negative charge and migrate towards the positive pole.

Analyze Protein B: The pI of Protein B is 6.4, which is equal to the pH of the buffer. Therefore, Protein B will have no net charge and will not migrate.

Analyze Proteins C and D: Protein C has a pI of 7.0, and Protein D has a pI of 9.2. Both are greater than 6.4, so they will have a positive charge and migrate towards the negative pole.