Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

5. Protein Techniques

Differential Centrifugation

Biochemistry

5. Protein Techniques

Differential Centrifugation

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Multiple Choice

What is the main purpose of differential centrifugation?

To make the cells dizzy before purifying proteins.

To separate out fractions of cell components with similar S values.

To pellet all unwanted proteins.

To fully purify the target protein of interest.

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Verified step by step guidance

Understand the concept of differential centrifugation: It is a technique used to separate cellular components based on their size and density by spinning them at various speeds.

Recognize that the main purpose of differential centrifugation is to separate cell components into fractions. This is achieved by subjecting the sample to increasing centrifugal forces, allowing components with different sedimentation coefficients (S values) to be separated.

Identify that components with similar S values will be grouped together in the same fraction, while those with different S values will be separated into different fractions.

Note that differential centrifugation is not used to fully purify proteins but rather to fractionate cell components, which can then be further purified using other techniques.

Clarify that the technique is not intended to pellet all unwanted proteins or make cells 'dizzy'; rather, it is a methodical approach to separate cellular components based on their physical properties.