According to the chart below, which one of the following enzymes has the strongest affinity for its substrate?

Indicate which region of the Enzyme Kinetics plot below best corresponds to each statement.

A) Initial reaction velocity is limited mainly by the [S] present: ______

B) Initial reaction velocity limited mainly by the [E] present: ______

C) The active site of an enzyme is most likely free/unoccupied: ______

D) The active site of an enzyme is most likely occupied by substrate: ______

E) This region includes the points corresponding to Km & ½Vmax: ______

Use the data in the following chart to determine the Km of the enzyme.

What is the initial velocity of a reaction when the concentration of substrate is set equal to the K_m?

Select the best description of the K _m.

Indicate which region of the Enzyme Kinetics plot below best corresponds to each statement.

A) Initial reaction velocity is limited mainly by the [S] present: ______

B) Initial reaction velocity limited mainly by the [E] present: ______

C) The active site of an enzyme is most likely free/unoccupied: ______

D) The active site of an enzyme is most likely occupied by substrate: ______

E) This region includes the points corresponding to Km & ½Vmax: ______