Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

5. Protein Techniques

Anion-Exchange Chromatography

Biochemistry

5. Protein Techniques

Anion-Exchange Chromatography

Previous problemNext problem

Struggling with Biochemistry?

Join thousands of students who trust us to help them ace their exams!Watch the first video

Multiple Choice

Use the chart to determine which tripeptide would elute last from an anion-exchange column at pH = 9.3.

Tyr-Lys-Met.

Gly-Pro-Arg.

Asp-Trp-Tyr.

Asp-His-Glu.

Previous problemNext problem

Verified step by step guidance

Understand that anion-exchange chromatography separates molecules based on their charge. At pH 9.3, molecules with a more negative charge will bind more strongly to the column and elute later.

Identify the ionizable groups in each tripeptide and their respective pKa values from the chart. For example, Asp (3.9), Glu (4.1), His (6), etc.

Calculate the net charge of each tripeptide at pH 9.3. Consider the ionization state of each ionizable group: if the pH is above the pKa, the group is deprotonated (negative for acidic groups, neutral for basic groups); if below, it is protonated (neutral for acidic groups, positive for basic groups).

For each tripeptide, sum the charges of the individual amino acids and the terminal groups (α-amino and α-carboxyl) to determine the overall charge at pH 9.3.

Compare the net charges: the tripeptide with the most negative net charge will elute last from the anion-exchange column. Based on the given answer, verify that Asp-His-Glu has the most negative charge at pH 9.3.