Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

6. Enzymes and Enzyme Kinetics

Shifting Lineweaver-Burk Plots

Biochemistry

6. Enzymes and Enzyme Kinetics

Shifting Lineweaver-Burk Plots

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Multiple Choice

Considering the Lineweaver-Burk plot below, which of the following enzymes would be better to use (converts more substrate to product) when the [S] = 0.001 M?

Enzyme A

Enzyme B

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Verified step by step guidance

Understand the Lineweaver-Burk plot: This plot is a double reciprocal graph of the Michaelis-Menten equation, where the y-axis represents \( \frac{1}{V_0} \) (the reciprocal of the initial velocity) and the x-axis represents \( \frac{1}{[S]} \) (the reciprocal of the substrate concentration).

Identify the lines for Enzyme A and Enzyme B: The plot shows two lines, one for each enzyme. The slope of each line is equal to \( \frac{K_m}{V_{max}} \), and the y-intercept is \( \frac{1}{V_{max}} \).

Determine \( V_{max} \) for each enzyme: The enzyme with the lower y-intercept has a higher \( V_{max} \), meaning it can convert more substrate to product at saturation. Compare the y-intercepts of Enzyme A and Enzyme B.

Evaluate the slope for each enzyme: The slope of the line is indicative of \( K_m \). A lower slope suggests a lower \( K_m \), which means the enzyme is more efficient at lower substrate concentrations.

Decide which enzyme is better at \( [S] = 0.001 \) M: At low substrate concentrations, the enzyme with the lower \( K_m \) (lower slope) will be more efficient. Compare the slopes of Enzyme A and Enzyme B to determine which enzyme is better suited for the given substrate concentration.