Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

5. Protein Techniques

Protein Purification

Biochemistry

5. Protein Techniques

Protein Purification

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Multiple Choice

Which of the following is likely the most appropriate meaning of protein purification?

Literally isolating only one single protein molecule.

Isolating a batch of the same exact protein molecule.

Isolating a batch of different protein molecules.

Isolating all the proteins of an organism's proteome.

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Verified step by step guidance

Understand the concept of protein purification: Protein purification is a process used to isolate a specific protein of interest from a complex mixture, such as a cell lysate or tissue extract.

Consider the goal of protein purification: The primary aim is to obtain a sample that contains a high concentration of the target protein with minimal contamination from other proteins or biomolecules.

Evaluate the options: The first option, 'Literally isolating only one single protein molecule,' is impractical and not the goal of protein purification. The third option, 'Isolating a batch of different protein molecules,' does not align with the goal of isolating a specific protein. The fourth option, 'Isolating all the proteins of an organism's proteome,' refers to proteomics, not purification of a single protein.

Focus on the second option: 'Isolating a batch of the same exact protein molecule' aligns with the goal of protein purification, which is to obtain a sample enriched in the target protein.

Conclude that the most appropriate meaning of protein purification is 'Isolating a batch of the same exact protein molecule,' as it reflects the process of obtaining a purified sample of a specific protein.