Multiple Choice
Calculate the degree of inhibition of an inhibitor on the free enzyme if the [I] = 3 μM and the KI = 6 μM.
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7. Enzyme Inhibition and Regulation
Degree of Inhibition
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Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
Calculate the degree of inhibition of an inhibitor on the ES-complex if the [I] = 8 μM and the K'I = 0.03 mM.
A
α = 0.87
B
α = 1.08
C
α = 1.27
D
α = 2.39
E
α = 0.27
Verified step by step guidance1
Understand that the degree of inhibition (α) is calculated using the formula: α = 1 + ([I] / K'I). This formula is derived from the concept of competitive inhibition in enzyme kinetics.
Convert the units of the inhibitor concentration [I] from micromolar (μM) to millimolar (mM) to match the units of K'I. Since 1 mM = 1000 μM, divide 8 μM by 1000 to get 0.008 mM.
Substitute the values into the formula: α = 1 + (0.008 mM / 0.03 mM).
Perform the division inside the parentheses: 0.008 mM / 0.03 mM.
Add 1 to the result of the division to find the degree of inhibition (α).
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Degree of Inhibition practice set
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