Multiple Choice
Why is a DNA polymerase from a thermophilic bacterium used in PCR?
18. Biotechnology
The Steps of PCR
Back18. Biotechnology
The Steps of PCR
- Multiple ChoiceA molecular biologist has isolated a short segment of DNA that she wants to replicate in vitro. First she heats the DNA, which separates the two strands, and then she adds __________.
- Multiple ChoiceIn the polymerase chain reaction (PCR) technique, a heating phase and a cooling phase alternate. An original sample of DNA would have to pass through how many total rounds of heating and cooling before a sample is increased eight times in quantity?
- Multiple Choice
Which of the following correctly lists the steps in order for one cycle of the polymerase chain reaction (PCR)?
- Multiple Choice
PCR is known for its power of amplifying a target DNA sequence at a high speed. Each cycle can double the
number of DNA molecules (target sequence). Which of the following is CORRECT regarding PCR?
- Multiple Choice
If you start with one double-stranded DNA molecule and you perform SIX cycles of PCR, how many double
stranded copies of the DNA will you have?
- Open QuestionAdd labels to the figure that follows, which illustrates the components in the PCR reaction.
- Open QuestionIf the sequence of DNA in Question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by -fold.
- Open QuestionIf the sequence of DNA in Question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by -fold.
- Open QuestionWhy was it important to include a positive control and a negative control in the PCR analysis?
- Open QuestionWhy was it important to include a positive control and a negative control in the PCR analysis?