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Why was it important to include a positive control and a negative control in the PCR analysis?
18. Biotechnology
The Steps of PCR
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Which of the following correctly lists the steps in order for one cycle of the polymerase chain reaction (PCR)?
A
Denature DNA; Add DNA polymerase; Anneal primers; Add dNTPs; Extend primers.
B
Anneal primers; Denature DNA; Extend primers.
C
Extend primers; Anneal primers; Denature DNA.
D
Denature DNA; Anneal primers; Extend primers utilizing a thermostable DNA polymerase.
Verified step by step guidance1
Understand that PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences.
The first step in a PCR cycle is 'Denaturation', where the double-stranded DNA is heated to around 94-98°C to separate it into two single strands.
The second step is 'Annealing', where the temperature is lowered to 50-65°C to allow primers to bind to the complementary sequences on the single-stranded DNA.
The third step is 'Extension', where the temperature is raised to around 72°C, and a thermostable DNA polymerase synthesizes a new DNA strand by adding dNTPs (deoxynucleotide triphosphates) to the primer.
Recognize that these steps are repeated for multiple cycles to exponentially amplify the target DNA sequence.
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