Multiple ChoiceIf you start with one double-stranded DNA molecule and you perform SIX cycles of PCR, how many doublestranded copies of the DNA will you have?
Open QuestionAdd labels to the figure that follows, which illustrates the components in the PCR reaction.
Open QuestionIf the sequence of DNA in Question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by -fold.
Open QuestionIf the sequence of DNA in Question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by -fold.
Open QuestionWhy was it important to include a positive control and a negative control in the PCR analysis?
Multiple ChoiceA molecular biologist has isolated a short segment of DNA that she wants to replicate in vitro. First she heats the DNA, which separates the two strands, and then she adds __________.
Multiple ChoiceIn the polymerase chain reaction (PCR) technique, a heating phase and a cooling phase alternate. An original sample of DNA would have to pass through how many total rounds of heating and cooling before a sample is increased eight times in quantity?