10. Transcription

Transcription in Eukaryotes

Multiple Choice
Which of the following polymerases is responsible for transcribing mRNA in eukaryotes?
Multiple Choice
Which of the following general transcription factors is responsible for binding to the TATA-Box
Multiple Choice
Which of the following modifications occurs to the RNA polymerase tail in order to trigger it to elongate the transcript?
Multiple Choice
Which of the following regulatory mechanisms regulates transcription from a great distance away from the gene?
Open Question
In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,How do we know that there is an association between disease susceptibility in humans and regulatory DNA sequences?
Open Question
In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,How do we know that eukaryotic transcription factors bind to DNA sequences at or near promoter regions?
Open Question
In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,How do we know that the orientation of promoters relative to the transcription start site is important while enhancers are orientation independent?
Open Question
In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,How do we know that promoter and enhancer sequences control the initiation of transcription in eukaryotes?
Open Question
In this chapter, we focused on how DNA is organized at the chromosomal level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions:What is the experimental basis for concluding that puffs in polytene chromosomes and loops in lampbrush chromosomes are areas of intense transcription of RNA?
Open Question
Write a short essay describing how cis-acting regulatory elements, activators, and chromatin modifiers are all coordinately involved in regulating transcription initiation.
Open Question
The diagram below shows a DNA duplex. The template strand is identified, as is the location of the nucleotide. If this region is a eukaryotic gene transcribed by RNA polymerase III, where are the promoter consensus sequences located? <>
Open Question
The diagram below shows a DNA duplex. The template strand is identified, as is the location of the nucleotide. Assume this region contains a gene transcribed to form mRNA in a eukaryote. Identify the location of the most common promoter consensus sequences. <>
Open Question
The following is a portion of an mRNA sequence: 3'-AUCGUCAUGCAGA-5' Identify the direction in which the promoter region for this gene will be located.
Open Question
The following is a portion of an mRNA sequence: 3'-AUCGUCAUGCAGA-5' Write out the sequence and polarity of the DNA duplex that encodes this mRNA segment. Label the template and coding DNA strands.
Open Question
The following is a portion of an mRNA sequence: 3'-AUCGUCAUGCAGA-5' During transcription, was the adenine at the left-hand side of the sequence the first or the last nucleotide used to build the portion of mRNA shown? Explain how you know.