In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,How do we know that eukaryotic transcription factors bind to DNA sequences at or near promoter regions?

In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,How do we know that there is an association between disease susceptibility in humans and regulatory DNA sequences?

In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,How do we know that the orientation of promoters relative to the transcription start site is important while enhancers are orientation independent?

In this chapter, we focused on how eukaryotic genes are regulated at the transcriptional level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,How do we know that promoter and enhancer sequences control the initiation of transcription in eukaryotes?

In this chapter, we focused on how DNA is organized at the chromosomal level. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions:What is the experimental basis for concluding that puffs in polytene chromosomes and loops in lampbrush chromosomes are areas of intense transcription of RNA?

Write a short essay describing how cis-acting regulatory elements, activators, and chromatin modifiers are all coordinately involved in regulating transcription initiation.