5. Genetics of Bacteria and Viruses
Bacterial Conjugation
- Open QuestionWith respect to F⁺ and F⁻ bacterial matings, answer the following questions:What is the genetic basis for a bacterium's being F⁺.
- Open QuestionWith respect to F⁺ and F⁻ bacterial matings, answer the following questions:How was it established that chromosome transfer was unidirectional?
- Open QuestionWith respect to F⁺ and F⁻ bacterial matings, answer the following questions:How was it established that physical contact between cells was necessary?
- Open QuestionBacteria transfer genes by conjugation, transduction, and transformation. Compare and contrast these mechanisms. In your answer, identify which if any processes involve homologous recombination and which if any do not.
- Open QuestionList all major differences between (a) the F⁺ x F⁻ and the Hfr x F⁻ bacterial crosses; and (b) the F⁺, F⁻, Hfr, and F' bacteria.
- Open Question
Explain the importance of the following features in conjugating donor bacteria:
pilin protein - Open QuestionExplain the importance of the following features in conjugating donor bacteria:the origin of transfer
- Open Question
Explain the importance of the following features in conjugating donor bacteria:
T strand DNA - Open Question
Explain the importance of the following features in conjugating donor bacteria:
relaxase - Open Question
Explain the importance of the following features in conjugating donor bacteria:
the relaxosome - Open Question
Explain the importance of the following features in conjugating donor bacteria:
homologous recombination - Open Question
Explain the importance of the following features in conjugating donor bacteria:
the conjugation pilus - Open QuestionDescribe the basis for chromosome mapping in the Hfr x F⁻ crosses.
- Open QuestionDescribe the origin of F' bacteria and merozygotes.
- Open Question
Among the mechanisms of gene transfer in bacteria, which one is capable of transferring the largest chromosome segment from donor to recipient? Which process generally transfers the smallest donor segments to the recipient? Explain your reasoning for both answers.