Describe the basis for chromosome mapping in the Hfr x F⁻ crosses.

Explain the importance of the following features in conjugating donor bacteria:

relaxase

Explain the importance of the following features in conjugating donor bacteria:

the relaxosome

Explain the importance of the following features in conjugating donor bacteria:

homologous recombination

Explain the importance of the following features in conjugating donor bacteria:

the conjugation pilus

Among the mechanisms of gene transfer in bacteria, which one is capable of transferring the largest chromosome segment from donor to recipient? Which process generally transfers the smallest donor segments to the recipient? Explain your reasoning for both answers.