Explain the importance of the following features in conjugating d...

Question

Explain the importance of the following features in conjugating donor bacteria:the origin of transfer

Accepted Answer

Hi, everyone. Let's look at our next problem. It says the blink serves as a recognition recognition site for the enzyme relaxation and initiates transfer of the F factor. And we have choice a attachment site. B origin of replication, C origin of transfer or D site specific recognition site. Well, when we think about beginning transfer of the F factor, um an enzyme relax. A, there's just a little bit of trickiness here between the origin of replication and the origin of transfer because we know that DNA replication will begin after the chromosome has been opened up at the F factor so that the strand starts being replicated. However, origin of replication is a more general term origin of replication can refer to just the bacterial chromosome to U Caro chromosomes. It's just the place where DNA replication starts, there can be multiple DNA replication site or multiple origins of replication. Um If you have a large amount of DNA as in new Caro cells, so it's not a specific enough term for what we're looking for, but we'll go ahead and eliminate choice B origin of transfer is what we're looking for. Because again, those F factors are used to be able to transfer DNA from one bacterial cell to another. And being that it's a circular DNA molecule, it needs to be nicked open on one of those DNA strands. So that as it starts replicating, you generate a single strand DNA molecule that can be transferred into another cell. So you have that enzyme relax ase that finds that origin of transfer recognition site nicks open one of the DNA strands and then that begins the process that will replicate this single strand of DNA that can transfer to another bacterial cell. Let's just look at our other two answer choices to understand why they're not correct choice. A, the attachment site that doesn't have to do with DNA. That's actually where a bacteria age attaches to a cell receptor on a bacterial cell and injects its DNA. So that's not what we're looking for. So we can eliminate choice A and then finally choice D A site specific specific recognition site. Well, this refers to DNA sites on two different strands of sufficient homology that DNA strand exchange can take place. So we've got this site where the two strands are similar enough that they can come together and the strands can actually be exchanged. And that's how the actual process of recombination takes place. But it's not where the transfer is initiated of the F factor and its associated genes. So choice D is not correct. So again, the choice C origin of transfer serves as a recognition site for the enzyme relaxation and initiates transfer of the X F factor. See you in the next video.

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Explain the importance of the following features in conjugating donor bacteria:the origin of transfer

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Bacterial Conjugation practice set