Open Question
How did Kornberg assess the fidelity of DNA polymerase I in copying a DNA template?
8. DNA Replication
Overview of DNA Replication
Back8. DNA Replication
Overview of DNA Replication
- Open QuestionWhich characteristics of DNA polymerase I raised doubts that its in vivo function is the synthesis of DNA leading to complete replication?
- Open QuestionDNA polymerase III is the main DNA-synthesizing enzyme in bacteria. Describe how it carries out its role of elongating a strand of DNA.
- Open QuestionKornberg showed that nucleotides are added to the 3' end of each growing DNA strand. In what way does an exposed 3'-OH group participate in strand elongation?
- Open QuestionWhat was the significance of the polA1 mutation?
- Open QuestionExplain how RNA participates in DNA replication.
- Open QuestionSummarize and compare the properties of DNA polymerase I, II, and III.
- Open Question
A sample of double-stranded DNA is found to contain 20% cytosine. Determine the percentage of the three other DNA nucleotides in the sample.
- Open QuestionList and describe the function of the ten subunits constituting DNA polymerase III. Distinguish between the holoenzyme and the core enzyme.
- Open Question
Bacterial DNA polymerase I and DNA polymerase III perform different functions during DNA replication.
If a strain of E. coli acquired a mutation that inactivated DNA polymerase III function, would the cell be able to replicate its DNA? Why or why not? - Open Question
Bacterial DNA polymerase I and DNA polymerase III perform different functions during DNA replication.
Identify the principal functions of each molecule. - Open Question
Bacterial DNA polymerase I and DNA polymerase III perform different functions during DNA replication.
If mutation inactivated DNA polymerase I in a strain of E. coli, would the cell be able to replicate its DNA? If so, what kind of abnormalities would you expect to find in the cell? - Open QuestionDistinguish between (a) unidirectional and bidirectional synthesis, and (b) continuous and discontinuous synthesis of DNA.
- Open Question
Diagram a replication fork in bacterial DNA and label the following structures or molecules.
a. DNA pol III
b. helicase
c. RNA primer
d. origin of replication
e. leading strand (label its polarity)
f. DNA pol I
g. topoisomerase
h. SSB protein
i. lagging strand (label its polarity)
j. primase
k. Okazaki fragment - Open QuestionList the proteins that unwind DNA during in vivo DNA synthesis. How do they function?