Which characteristics of DNA polymerase I raised doubts that its in vivo function is the synthesis of DNA leading to complete replication?

Predict the results of the experiment by Taylor, Woods, and Hughes if replication were (a) conservative and (b) dispersive.

What are the requirements for in vitro synthesis of DNA under the direction of DNA polymerase I?

In Kornberg's initial experiments, it was rumored that he grew E. coli in Anheuser-Busch beer vats. (Kornberg was working at Washington University in St. Louis.) Why do you think this might have been helpful to the experiment?

How did Kornberg assess the fidelity of DNA polymerase I in copying a DNA template?

DNA polymerase III is the main DNA-synthesizing enzyme in bacteria. Describe how it carries out its role of elongating a strand of DNA.

Kornberg showed that nucleotides are added to the 3' end of each growing DNA strand. In what way does an exposed 3'-OH group participate in strand elongation?

What was the significance of the polA1 mutation?

Explain how RNA participates in DNA replication.