12. Gene Regulation in Prokaryotes

Lac Operon

Open Question
The CAP binding site in the lac promoter is the location of positive regulation of gene expression for the operon. Identify what binds at this site to produce positive regulation, under what circumstances binding occurs, and how binding exerts a positive effect.
Multiple Choice
Which of the following is not a part of an operon?
Multiple Choice
The lac operon encodes genes that are responsible for what?
Multiple Choice
What happens to the lac operon when lactose concentration is high?
Multiple Choice
What happens to the lac operon when the CAP/cAMP complex binds to the CAP binding site?
Multiple Choice
Glucose concentration can regulate the lac operon?
Open Question
What are the advantages and disadvantages of using GFP versus lacZ as a reporter gene in mice, C. elegans, and Drosophila?
Open Question
Bacterial genomes frequently contain groups of genes organized into operons. What is the biological advantage of operons to bacteria? Identify the regulatory components you would expect to find in an operon. How are the expressed genes of an operon usually arranged?
Open Question
In this chapter, we focused on the regulation of gene expression in bacteria. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?How do we know that bacteria regulate the expression of certain genes in response to the environment?
Open Question
In this chapter, we focused on the regulation of gene expression in bacteria. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?How do we know that the lac repressor is a protein?
Open Question
In this chapter, we focused on the regulation of gene expression in bacteria. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?What led researchers to conclude that a repressor molecule regulates the lac operon?
Open Question
In this chapter, we focused on the regulation of gene expression in bacteria. Along the way, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?What evidence established that lactose serves as the inducer of a gene whose product is related to lactose metabolism?
Open Question
Transcriptional regulation of operon gene expression involves the interaction of molecules with one another and of regulatory molecules with segments of DNA. In this context, define and give an example of each of the following:negative regulation
Open Question
Transcriptional regulation of operon gene expression involves the interaction of molecules with one another and of regulatory molecules with segments of DNA. In this context, define and give an example of each of the following:allostery
Open Question
Transcriptional regulation of operon gene expression involves the interaction of molecules with one another and of regulatory molecules with segments of DNA. In this context, define and give an example of each of the following:positive regulation