Multiple Choice
Human DNA cut with restriction enzyme A can be joined to:
18. Biotechnology
Steps to DNA Cloning
Back18. Biotechnology
Steps to DNA Cloning
- Multiple Choice
Each restriction enzyme has a specific sequence of nucleotides where it cuts the DNA. These sequences of DNA are unique to each restriction enzyme and are known as:
- Multiple Choice
The single-stranded ends of DNA molecules can be joined together by:
- Multiple Choice
The process of using DNA from one organism to alter the characteristics of another is called:
- Multiple Choice
An organism which has foreign genes incorporated into its genomes is known as a:
- Multiple Choice
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases).
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligase to seal plasmid DNA to non-plasmid DNA. - Multiple ChoiceWhat two enzymes are needed to produce recombinant DNA?
- Multiple ChoiceWhich arrangement of the following four enzymes represents the order in which they would be used in a typical gene-cloning experiment resulting in the insertion of a cDNA into a bacterial plasmid? Begin with the gene's mRNA transcript.
- Multiple Choice
What is the enzymatic function of restriction enzymes?
- Open QuestionWhat do restriction endonucleases do?
- Open QuestionWhat do restriction endonucleases do?
- Open QuestionWhat is a plasmid?a. an organelle found in many bacteria and certain eukaryotesb. a circular DNA molecule that replicates independently of the main chromosome(s)c. a type of virus that has a DNA genome and infects certain types of human cells, including lung and respiratory tract tissued. a type of virus that has an RNA genome, codes for reverse transcriptase, and inserts a cDNA copy of its genome into cells
- Open QuestionAfter finding a gene that causes a disease, researchers often introduce the defective allele into mice to create an animal model of the disease. Why are these models valuable?a. They allow the testing of potential drug therapies without endangering human patients.b. They allow the sequencing of the mutant allele.c. They allow the production of large quantities of the defective gene product, usually a protein.d. They allow the study of how the gene was transmitted from parents to offspring.
- Open QuestionDNA technology has many medical applications. Which of the following is not done routinely at present?a. production of hormones for treating diabetes and dwarfismb. analysis of gene expression for more informed cancer treatmentsc. gene editing by the CRISPR-Cas9 system in viable human embryos to correct genetic diseasesd. prenatal identification of genetic disease alleles
- Open QuestionWhen a typical restriction enzyme cuts a DNA molecule, the cuts are uneven, giving the DNA fragments single-stranded ends. These ends are useful in recombinant DNA work becausea. they enable a cell to recognize fragments produced by the enzyme.b. they serve as starting points for DNA replication.c. the fragments will bond to other fragments with complementary ends.d. they enable researchers to use the fragments as molecular probes.