What is the enzymatic function of restriction enzymes?

What two enzymes are needed to produce recombinant DNA?

Which arrangement of the following four enzymes represents the order in which they would be used in a typical gene-cloning experiment resulting in the insertion of a cDNA into a bacterial plasmid? Begin with the gene's mRNA transcript.

What do restriction endonucleases do?

What do restriction endonucleases do?

What is a plasmid?a. an organelle found in many bacteria and certain eukaryotesb. a circular DNA molecule that replicates independently of the main chromosome(s)c. a type of virus that has a DNA genome and infects certain types of human cells, including lung and respiratory tract tissued. a type of virus that has an RNA genome, codes for reverse transcriptase, and inserts a cDNA copy of its genome into cells

After finding a gene that causes a disease, researchers often introduce the defective allele into mice to create an animal model of the disease. Why are these models valuable?a. They allow the testing of potential drug therapies without endangering human patients.b. They allow the sequencing of the mutant allele.c. They allow the production of large quantities of the defective gene product, usually a protein.d. They allow the study of how the gene was transmitted from parents to offspring.