In hypothesis testing involving two samples, the primary focus shifts from a single mean to the difference between two sample means. The process remains fundamentally similar to that of a one-sample test, involving the formulation of hypotheses, calculation of test statistics, determination of p-values, and drawing conclusions.
The initial step is to establish the null hypothesis, which posits that the two means are equal: H_0: \mu_1 = \mu_2. This can also be expressed as H_0: \mu_1 - \mu_2 = 0. The alternative hypothesis, which indicates a difference, is typically framed as H_a: \mu_1 \neq \mu_2, suggesting a two-tailed test.
Before proceeding, it is essential to verify certain conditions: the samples must be random and independent, the population standard deviations (\sigma_1 and \sigma_2) are unknown and not assumed to be equal, and the samples should either be normally distributed or sufficiently large.
The test statistic for a two-sample t-test is calculated using the formula:
t = \frac{(\bar{x}_1 - \bar{x}_2) - (\mu_1 - \mu_2)}{\sqrt{\frac{s_1^2}{n_1} + \frac{s_2^2}{n_2}}}
In this formula, \bar{x}_1 and \bar{x}_2 represent the sample means, s_1 and s_2 are the sample standard deviations, and n_1 and n_2 are the sample sizes. The difference in population means is assumed to be zero under the null hypothesis.
After calculating the t-score, the next step is to determine the p-value, which indicates the probability of observing the test statistic under the null hypothesis. For two samples, the degrees of freedom can be approximated by taking the smaller of the two sample sizes minus one: df = min(n_1, n_2) - 1.
Once the p-value is obtained, it is compared to the significance level (alpha). If the p-value is less than alpha, the null hypothesis is rejected, indicating sufficient evidence to support the alternative hypothesis. For example, if the p-value is 0.0005 and alpha is set at 0.05, the conclusion would be to reject the null hypothesis, suggesting a significant difference in means.
In summary, conducting a two-sample hypothesis test involves establishing hypotheses about the means, ensuring the validity of assumptions, calculating the test statistic, determining the p-value, and making a conclusion based on the comparison with the significance level. This structured approach allows for a clear understanding of differences between groups, such as in studies comparing mean resting heart rates between males and females.