Human DNA cut with restriction enzyme A can be joined to:
18. Biotechnology
Steps to DNA Cloning
- Multiple Choice
- Multiple Choice
Each restriction enzyme has a specific sequence of nucleotides where it cuts the DNA. These sequences of DNA are unique to each restriction enzyme and are known as:
- Multiple Choice
The single-stranded ends of DNA molecules can be joined together by:
- Multiple Choice
The process of using DNA from one organism to alter the characteristics of another is called:
- Multiple Choice
An organism which has foreign genes incorporated into its genomes is known as a:
- Multiple Choice
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases).
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligase to seal plasmid DNA to non-plasmid DNA. - Multiple ChoiceWhat two enzymes are needed to produce recombinant DNA?
- Multiple ChoiceWhich arrangement of the following four enzymes represents the order in which they would be used in a typical gene-cloning experiment resulting in the insertion of a cDNA into a bacterial plasmid? Begin with the gene's mRNA transcript.