17. Biotechnology

Human DNA cut with restriction enzyme A can be joined to:

What is the enzymatic function of restriction enzymes?

Each restriction enzyme has a specific sequence of nucleotides where it cuts the DNA. These sequences of DNA are unique to each restriction enzyme and are known as:

The single-stranded ends of DNA molecules can be joined together by:

The process of using DNA from one organism to alter the characteristics of another is called:

An organism which has foreign genes incorporated into its genomes is known as a:

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases).
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligase to seal plasmid DNA to non-plasmid DNA.

Match the following choices to the statements in questions 7 through 10.

a. antisense

b. clone

c. library

d. Southern blot

e. vector

A population of cells carrying a desired plasmid.