17. Biotechnology

Which of the following correctly lists the steps in order for one cycle of the polymerase chain reaction (PCR)?

Why is a DNA polymerase from a thermophilic bacterium used in PCR?

PCR is known for its power of amplifying a target DNA sequence at a high speed. Each cycle can double the number of DNA molecules (target sequence). Which of the following is CORRECT regarding PCR?

If you start with one double-stranded DNA molecule and you perform SIX cycles of PCR, how many double- stranded copies of the DNA will you have?

Suppose you want multiple copies of a gene you have synthesized. How would you obtain the necessary copies by cloning? By PCR?

You have a small gene that you want replicated by PCR. You add fluorescent dye-labeled nucleotides to the PCR thermal cycler. After three replication cycles, what percentage of the DNA single strands will fluoresce?

a. 0%

b. 12.5%

c. 50%

d. 87.5%

e. 100%

Use the following choices to answer questions 9 and 10:

a. C. trachomatis

b. E. coli

c. Mycobacterium hominis

d. S. saprophyticus

In cases of NGU, diagnosis is made using PCR to detect microbial DNA.