17. Biotechnology

A southern blotting technique is used to detect a specific ______ sequence from a blood or tissue sample.

A _____ is a single-stranded DNA molecule used in hybridization reactions to detect the presence of a particular gene in an assortment of DNA fragments.
(Hybridization Reactions:reactions that combine two complementary single-stranded DNA molecules)

A geneticist wants to see if her patient has Gene X. The geneticist takes a blood sample from her patient and prepares a southern blot. How will the geneticist know if her patient possesses Gene X?

Place the following steps of Southern Blotting in the correct order.
a) ______:Filter paper is incubated with the labeled DNA probe which anneals to the ssDNA fragments.
b) ______:Analyze gel to determine the presence of the DNA sequence of interest.
c) ______:Separate DNA fragments by size using gel electrophoresis.
d) ______:Denature DNA by soaking gel in a basic solution.
e) ______:Fragment unknown DNA sample(s) using restriction enzymes.

By analyzing the Southern Blot results below, which of the samples contains the gene of interest?

Here is some additional information on the organisms in question 1:

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Which of these organisms are most closely related? Compare this answer with your response to review question 1.