10. Dynamics of Microbial Growth

Direct microscopic cell counting is a method where the microbiologist counts the number of microbes in a fraction of the microbial culture to:

Determining the number of cells within a culture is important. However, counting each and every cell with the human eye is tedious and extremely time consuming. Which of these is a method of cell counting that does not require a scientist to count each and every cell?

Match the term to the proper description(s). A term can have more than one description. <IMAGE>

Choose the false statement about turbidity as an enumeration method.

a. It is an indirect enumeration method.

b. It is performed using a spectrophotometer.

c. It must be done using a liquid culture.

d. It differentiates between live and dead cells.

e. It is a rapid enumeration method.

Which direct enumeration method differentiates living from nonliving cells?

a. Manual cell counts

b. Measuring dry weight of cells

c. Viable plate count

d. Measuring biochemical activity

e. Coulter counter

A Coulter counter is a(n)_______.

a. statistical estimation using 15 dilution tubes and a table of numbers to estimate the number of bacteria per milliliter

b. indirect method of counting microorganisms

c. device that directly counts microbes as they pass through a tube in front of an electronic detector

d. device that directly counts microbes that are differentially stained with fluorescent dyes

If there are 47 cells in 1 μl of sewage, how many cells are there in a liter?

List five direct methods of counting microbes.