Table of contents

1. Introduction to Microbiology3h 22m
2. Disproving Spontaneous Generation1h 18m
3. Chemical Principles of Microbiology3h 38m
4. Water1h 28m
5. Molecules of Microbiology2h 23m
6. Cell Membrane & Transport3h 28m
7. Prokaryotic Cell Structures & Functions5h 52m
8. Eukaryotic Cell Structures & Functions2h 18m
9. Microscopes2h 46m
10. Dynamics of Microbial Growth4h 36m
11. Controlling Microbial Growth4h 10m
12. Microbial Metabolism5h 16m
13. Photosynthesis2h 31m
14. DNA Replication2h 25m
15. Central Dogma & Gene Regulation7h 14m
16. Microbial Genetics4h 44m
17. Biotechnology3h 0m
18. Viruses, Viroids, & Prions4h 56m
19. Innate Immunity7h 15m
20. Adaptive Immunity7h 14m
21. Principles of Disease6h 57m

16. Microbial Genetics

Mutant Detection

Microbiology

16. Microbial Genetics

Mutant Detection

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Multiple Choice

A scientist has a population of His+ prototroph and His- auxotroph cells and wants to select and separate the auxotrophs from the prototrophs. Which form of selection should the scientist use?

Indirect selection.

Auxotroph selection.

Direct selection.

Any of the above forms of selection can be used.

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Verified step by step guidance

Understand the difference between prototrophs and auxotrophs: Prototrophs can grow on minimal media without additional nutrients, while auxotrophs require specific supplements to grow.

Recognize that the goal is to select and separate His- auxotrophs from His+ prototrophs. This means you need a method that allows only the auxotrophs to be identified or isolated.

Consider the concept of indirect selection: This involves using conditions where only the desired mutants (auxotrophs) are unable to grow, while the prototrophs can grow. This is often done by replica plating.

Understand direct selection: This method allows only the desired mutants to grow, which is not applicable here since auxotrophs cannot grow without histidine.

Conclude that indirect selection is the appropriate method because it allows the identification of auxotrophs by their inability to grow on minimal media, while prototrophs will grow, thus separating the two populations.