Table of contents

1. Introduction to Microbiology3h 22m
2. Disproving Spontaneous Generation1h 18m
3. Chemical Principles of Microbiology3h 38m
4. Water1h 28m
5. Molecules of Microbiology2h 23m
6. Cell Membrane & Transport3h 28m
7. Prokaryotic Cell Structures & Functions5h 52m
8. Eukaryotic Cell Structures & Functions2h 18m
9. Microscopes2h 46m
10. Dynamics of Microbial Growth4h 36m
11. Controlling Microbial Growth4h 10m
12. Microbial Metabolism5h 16m
13. Photosynthesis2h 31m
14. DNA Replication2h 25m
15. Central Dogma & Gene Regulation7h 14m
16. Microbial Genetics4h 44m
17. Biotechnology3h 0m
18. Viruses, Viroids, & Prions4h 56m
19. Innate Immunity7h 15m
20. Adaptive Immunity7h 14m
21. Principles of Disease6h 57m

16. Microbial Genetics

Mutant Detection

Microbiology

16. Microbial Genetics

Mutant Detection

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Multiple Choice

What types of bacteria will be growing on the nutrient agar and minimal media at the end of a replica plating experiment?

The nutrient agar will possess prototroph bacteria, while the minimal media will possess auxotroph bacteria.

The nutrient agar will possess both prototroph & auxotroph bacteria, while the minimal media will possess prototroph bacteria.

The nutrient agar will possess auxotroph bacteria, while the minimal media will possess the prototroph bacteria.

The nutrient agar will possess auxotroph bacteria, while the minimal media will possess both prototroph & auxotroph bacteria.

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Verified step by step guidance

Understand the definitions: Prototrophs are bacteria that can grow on minimal media because they can synthesize all the compounds they need from basic nutrients. Auxotrophs require additional nutrients that they cannot synthesize themselves.

Review the concept of replica plating: This technique is used to transfer colonies of bacteria from one plate to another, maintaining the same spatial arrangement. It helps identify mutants, such as auxotrophs, by comparing growth on different media.

Consider the growth conditions: On nutrient agar, which is rich in nutrients, both prototrophs and auxotrophs can grow because all necessary nutrients are provided.

Analyze the minimal media conditions: Minimal media lacks certain nutrients, so only prototrophs, which can synthesize all necessary compounds, will grow. Auxotrophs will not grow because they lack the ability to synthesize one or more essential compounds.

Conclude based on the above analysis: The nutrient agar will support the growth of both prototroph and auxotroph bacteria, while the minimal media will only support the growth of prototroph bacteria.