Multiple Choice
Which of the following is NOT required for the reactions in dideoxy sequencing?
17. Biotechnology
Dideoxy Sequencing
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The final step in a Sanger DNA sequencing reaction is to run the DNA fragments on a gel. What purpose does this serve?
A
It adds ddNTP to the end of each DNA fragment.
B
It changes the length of the DNA fragments.
C
It separates DNA fragments based on their sequence.
D
It separates DNA fragments generated during the sequencing reaction based on one nucleotide differences in their size.
Verified step by step guidance1
Understand the purpose of Sanger sequencing: It is a method used to determine the nucleotide sequence of DNA by incorporating chain-terminating nucleotides (ddNTPs) during DNA synthesis.
Recognize the role of ddNTPs: These modified nucleotides terminate DNA strand elongation, resulting in DNA fragments of varying lengths, each ending with a ddNTP.
Learn about gel electrophoresis: This technique is used to separate DNA fragments based on size by applying an electric field to a gel matrix, allowing smaller fragments to move faster than larger ones.
Identify the purpose of running DNA fragments on a gel: The gel electrophoresis step in Sanger sequencing is crucial for separating DNA fragments that differ by a single nucleotide, enabling the determination of the DNA sequence.
Conclude the process: By analyzing the pattern of separated DNA fragments on the gel, researchers can deduce the sequence of the original DNA strand, as each fragment corresponds to a specific nucleotide position.
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