Table of contents

1. Introduction to Microbiology3h 22m
2. Disproving Spontaneous Generation1h 18m
3. Chemical Principles of Microbiology3h 38m
4. Water1h 28m
5. Molecules of Microbiology2h 23m
6. Cell Membrane & Transport3h 28m
7. Prokaryotic Cell Structures & Functions5h 52m
8. Eukaryotic Cell Structures & Functions2h 18m
9. Microscopes2h 46m
10. Dynamics of Microbial Growth4h 36m
11. Controlling Microbial Growth4h 10m
12. Microbial Metabolism5h 16m
13. Photosynthesis2h 31m
14. DNA Replication2h 25m
15. Central Dogma & Gene Regulation7h 14m
16. Microbial Genetics4h 44m
17. Biotechnology3h 0m
18. Viruses, Viroids, & Prions4h 56m
19. Innate Immunity7h 15m
20. Adaptive Immunity7h 14m
21. Principles of Disease6h 57m

17. Biotechnology

Dideoxy Sequencing

Microbiology

17. Biotechnology

Dideoxy Sequencing

Previous problemNext problem

Struggling with Microbiology?

Join thousands of students who trust us to help them ace their exams!Watch the first video

Multiple Choice

According to the gel below, which of the following is the correct sequence on the unknown DNA molecule?

5 '- GCGATGCCAT - 3'

5' - ATGGCATCGC - 3'

5' - TACCGTAGCG - 3'

5' - CGCTACGGTA - 3'

None of the above are the correct sequence.

Previous problemNext problem

Verified step by step guidance

Examine the gel electrophoresis image provided. The gel shows four lanes labeled A, C, G, and T, which correspond to the four nucleotides adenine, cytosine, guanine, and thymine, respectively.

Identify the bands in each lane. Each band represents a DNA fragment that ends with the nucleotide corresponding to the lane it is in. The position of the band indicates the length of the fragment.

Starting from the bottom of the gel (which represents the 5' end of the sequence), read the sequence by noting the lane in which each band appears. This will give you the sequence of the DNA strand from 5' to 3'.

Write down the sequence as you read from the bottom to the top of the gel. For example, if the first band from the bottom is in the T lane, the first nucleotide is T, and so on.

Compare the sequence you have determined with the options provided in the problem. Check if any of the options match the sequence you have derived from the gel.