Multiple Choice
There are three major classes of light microscopes. Which class of light microscopes specializes in visualizing the difference in color intensity between the specimen and its background?
9. Microscopes
Introduction to Light Microscopy
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Some light microscopes are able to create 3-dimensional images of the microscopic specimen. Which class(es) of light microscopes are the 3-D microscopes found in?
A
Bright-field microscopes.
B
Light microscopes that increase contrast.
C
Light microscopes that detect fluorescence.
D
Both A and B.
E
Both B and C.
F
Both A and C.
Verified step by step guidance1
Understand the different classes of light microscopes: Bright-field, contrast-enhancing, and fluorescence-detecting microscopes.
Bright-field microscopes are the most common type and typically produce 2-dimensional images by transmitting light through the specimen.
Contrast-enhancing microscopes, such as phase-contrast and differential interference contrast (DIC) microscopes, can create 3-dimensional images by enhancing the contrast of transparent specimens.
Fluorescence microscopes use fluorescent dyes and special lighting to visualize specimens, and some advanced types, like confocal microscopes, can produce 3-dimensional images by scanning the specimen at different depths.
Conclude that the classes of light microscopes capable of producing 3-dimensional images include those that increase contrast and those that detect fluorescence, which corresponds to options B and C.
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