Multiple Choice
The __________ method is used to isolate microbial colonies while the __________ method is used to count the number of viable microbial cells or colonies.
10. Dynamics of Microbial Growth
Plating Methods
Struggling with Microbiology?
Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
In the streak-plate method, what is done with the inoculating loop after each streak?
A
It is discarded and another one is used.
B
It is re-introduced to the culture stock.
C
It is disinfected with an antiseptic.
D
It is flamed until the loop is red with heat.
Verified step by step guidance1
Understand the purpose of the streak-plate method: It is used to isolate individual colonies from a mixed culture by spreading the bacteria over the surface of an agar plate.
Recognize the importance of sterilizing the inoculating loop: This prevents contamination and ensures that only the bacteria from the initial streak are spread in subsequent streaks.
After each streak, the inoculating loop must be sterilized by flaming: This involves passing the loop through a flame until it is red hot, effectively killing any remaining bacteria.
Allow the loop to cool before using it again: This prevents killing the bacteria you are trying to streak and ensures proper transfer.
Repeat the streaking process: After sterilizing and cooling the loop, proceed with the next streak, ensuring to overlap slightly with the previous streak to encourage isolated colony formation.
Related Videos
Related Practice
Plating Methods practice set
Problem sets built by lead tutorsExpert video explanations