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Multiple Choice
Which of the following is the correct transcript of mRNA for the following DNA template? DNA Template:3'-ATGAAGCCGAGTCAT-5'
A
3'-TACTTCGGCTCAGTA-5'
B
3'-AUGACUCGGCUUCAU-5'
C
3'-UACUUCGGCUCAGUA-5'
D
3'-ATGACTCGGCTTCAT-5'
Verified step by step guidance
1
Understand the directionality of the DNA template strand. The given DNA template is in the 3' to 5' direction, which is the direction in which RNA polymerase reads the template to synthesize mRNA.
Identify the complementary base pairing rules for transcription. In RNA, adenine (A) pairs with uracil (U), thymine (T) pairs with adenine (A), cytosine (C) pairs with guanine (G), and guanine (G) pairs with cytosine (C).
Transcribe the DNA template strand into mRNA. Starting from the 3' end of the DNA template, apply the base pairing rules to each nucleotide to form the mRNA strand in the 5' to 3' direction.
Check the sequence of the transcribed mRNA. Ensure that the mRNA sequence is complementary to the DNA template strand and follows the correct base pairing rules.
Compare the transcribed mRNA sequence with the given options to determine which one matches the correct mRNA transcript.