Textbook Question
Radiation is frequently used as part of the treatment of cancer. The radiation works by damaging DNA and components of the cell. Under what circumstances do you think radiation treatment is a good choice to treat cancer?
Ch. 14 - Analysis of Gene Function via Forward Genetics and Reverse Genetics
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172
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Table of contents
- Ch. 1 - The Molecular Basis of Heredity, Variation, and Evolution64
- Ch. 2 - Transmission Genetics144
- Ch. 3 - Cell Division and Chromosome Heredity81
- Ch. 4 - Gene Interaction87
- Ch. 5 - Genetic Linkage and Mapping in Eukaryotes103
- Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages73
- Ch. 7 - DNA Structure and Replication71
- Ch. 8 - Molecular Biology of Transcription and RNA Processing79
- Ch. 9 - The Molecular Biology of Translation110
- Ch. 10 - Eukaryotic Chromosome Abnormalities and Molecular Organization100
- Ch. 11 - Gene Mutation, DNA Repair, and Homologous Recombination86
- Ch. 12 - Regulation of Gene Expression in Bacteria and Bacteriophage90
- Ch. 13 - Regulation of Gene Expression in Eukaryotes38
- Ch. 14 - Analysis of Gene Function via Forward Genetics and Reverse Genetics72
- Ch. 15 - Recombinant DNA Technology and Its Applications69
- Ch. 16 - Genomics: Genetics from a Whole-Genome Perspective49
- Ch. 17 - Organelle Inheritance and the Evolution of Organelle Genomes27
- Ch. 18 - Developmental Genetics43
- Ch. 19 - Genetic Analysis of Quantitative Traits66
- Ch. 20 - Population Genetics and Evolution at the Population, Species, and Molecular Levels105
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
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Sanders 3rd EditionCh. 14 - Analysis of Gene Function via Forward Genetics and Reverse GeneticsProblem 6
Sanders 3rd EditionCh. 14 - Analysis of Gene Function via Forward Genetics and Reverse GeneticsProblem 6Chapter 14, Problem 6
You have cloned the mouse ortholog of the gene associated with human Huntington disease (HD) and wish to examine its expression in mice. Outline the approaches you might take to examine the temporal and spatial expression pattern at the cellular level.
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Extract RNA from various tissues of the mouse at different developmental stages to analyze the temporal and spatial expression of the gene. Use reverse transcription to convert RNA into complementary DNA (cDNA) for further analysis.
Perform in situ hybridization using a labeled RNA probe complementary to the mouse ortholog of the Huntington disease gene. This technique will allow visualization of the gene's expression at the cellular level in tissue sections.
Use quantitative PCR (qPCR) to measure the levels of gene expression in different tissues and at different developmental stages. This will provide quantitative data on the temporal expression pattern.
Perform immunohistochemistry (IHC) using an antibody specific to the protein encoded by the mouse ortholog of the Huntington disease gene. This will allow visualization of the protein's localization and expression at the cellular level.
Generate a transgenic mouse model with a reporter gene (e.g., GFP or LacZ) under the control of the promoter of the mouse ortholog. This will enable real-time observation of the gene's expression pattern in live tissues and at different developmental stages.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Orthologs
Orthologs are genes in different species that evolved from a common ancestral gene through speciation. They typically retain the same function across species, making them valuable for studying gene function and disease mechanisms. In this context, the mouse ortholog of the Huntington disease gene allows researchers to investigate the gene's role in a model organism that shares similarities with humans.
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Overview
Gene Expression Analysis
Gene expression analysis involves measuring the activity of genes to understand their function and regulation. Techniques such as quantitative PCR, in situ hybridization, and RNA sequencing can be employed to assess when (temporal) and where (spatial) a gene is expressed in tissues or cells. This information is crucial for linking gene expression patterns to specific biological processes or diseases.
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Cellular Localization Techniques
Cellular localization techniques, such as immunohistochemistry and fluorescence in situ hybridization (FISH), are used to visualize the presence and distribution of proteins or RNA within cells. These methods allow researchers to determine the specific cell types and regions in which the gene of interest is active, providing insights into its functional role in development and disease pathology.
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Related Practice
Textbook Question
Explain the following processes involving chromosome mutations and cancer development.
How the chromosome mutation producing Burkitt lymphoma generates the disease.
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Explain the following processes involving chromosome mutations and cancer development.
How the chromosome mutation producing the Philadelphia chromosome leads to CML.
Textbook Question
What are the advantages and disadvantages of using insertion alleles versus alleles generated by chemicals (as in TILLING) in reverse genetic studies?
Textbook Question
Radiation is frequently used as part of the treatment of cancer. The radiation works by damaging DNA and components of the cell. Is there a risk of damage to noncancer cells?
Textbook Question
Radiation is frequently used as part of the treatment of cancer. The radiation works by damaging DNA and components of the cell. How can radiation treatment control or cure cancer?