Photosynthesis is a vital process that occurs in the chloroplasts of plants, where it is divided into two main stages: the light reactions and the Calvin cycle. The light reactions take place in the thylakoids, which are the green, pancake-like structures within the chloroplasts. During this stage, photons of light are absorbed by pigments in two photosystems, known as photosystem II and photosystem I. This energy is used to split water molecules, a process that generates oxygen gas as a byproduct, which can either be utilized by the plant for aerobic cellular respiration or released into the atmosphere.
The light reactions also produce NADPH, an electron carrier, and ATP, which are essential for the next stage of photosynthesis. A mnemonic to remember the sequence of events in the light reactions is "Luke and Ryan," where Luke represents photosystem II, and Ryan represents photosystem I. The electrons that are released during the splitting of water travel through an electron transport chain, ultimately leading to the reduction of NADP+ to NADPH and the production of ATP through chemiosmosis.
The Calvin cycle, occurring in three phases, utilizes the ATP and NADPH generated from the light reactions. The first phase, carbon fixation, involves the enzyme rubisco, which attaches carbon dioxide to ribulose bisphosphate (RuBP). This reaction produces 3-phosphoglycerate (PGA), which is then converted into glyceraldehyde-3-phosphate (G3P) in the second phase. G3P is a precursor for glucose synthesis. The final phase, RuBP regeneration, ensures that RuBP is available for the cycle to continue. A helpful way to remember the reactants of the Calvin cycle is "Calvin's can of sugar," where the letters represent carbon dioxide, ATP, and NADPH.
Under normal conditions, photosynthesis occurs with open stomata, allowing for gas exchange. However, in hot conditions, stomata may close to prevent dehydration, leading to a process called photorespiration, which is inefficient and primarily affects C3 plants. To mitigate this, some plants have evolved adaptations. C4 plants separate the light reactions and Calvin cycle spatially in different cell types, while CAM plants fix carbon at different times of the day, allowing them to thrive in arid environments.
Understanding these processes is crucial for grasping how plants convert light energy into chemical energy, ultimately supporting life on Earth through the production of oxygen and organic compounds.