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Multiple Choice
A gene family has been identified that has undergone extensive duplication in humans but is present in only one or a few copies in other primates or other mammals. What experimental approaches could be used to determine the function of this gene and its significance in human evolution?
A
Reduce the number of copies of this gene in a human subject.
B
Determine the timing and location of expression of these genes during embryonic development in fruit flies.
C
Introduce these genes into yeast and study the phenotype of the resulting cells.
D
All of the listed responses are correct.
E
Create knockout mice to determine their phenotype.
Verified step by step guidance
1
Identify the gene family of interest that has undergone duplication in humans. This involves using genomic databases to compare the number of gene copies in humans versus other primates or mammals.
Use bioinformatics tools to analyze the gene sequence and predict potential functions based on similarity to known genes. This can provide initial insights into the gene's role.
Create knockout mice by using techniques such as CRISPR-Cas9 to specifically disrupt the gene of interest. This allows researchers to observe the effects of the gene's absence on the organism's phenotype.
Observe and document the phenotypic changes in the knockout mice. This includes monitoring physical, behavioral, and physiological traits to infer the gene's function.
Compare the phenotypic data from knockout mice with the known traits of humans and other mammals to understand the gene's significance in human evolution. This can help determine if the gene contributes to unique human traits.