What is recombinant DNA made of?

Which of the following is not true of cDNA produced using human brain tissue as the starting material?a. It can be amplified by the polymerase chain reaction.b. It was produced from pre-mRNA using reverse transcriptase.c. It can be labeled and used as a probe to detect genes expressed in the brain.d. It lacks the introns of the pre-mRNA.

Recombinant DNA techniques are used to custom-build bacteria for two main purposes: to obtain multiple copies of certain genes and to obtain useful proteins produced by certain genes. Give an example of each of these applications in medicine and agriculture.

Gene cloning is crucial to any application involving one gene because __________.

Bacteria use restriction enzymes to __________.

Which of the following enzymes could seal a nick in one strand of a double-stranded DNA molecule by creating a sugar-phosphate bond between the adjacent, unjoined nucleotides?

The efficiency of cloning, and the ability to generate healthy cloned animals, has been largely hampered by the difficulty of __________.