Multiple Choice
The unwinding of DNA at the replication fork causes twisting and strain in the DNA ahead of the fork, which is relieved by an enzyme called __________.
14. DNA Synthesis
Steps of DNA Replication
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Which of the following enzymes is responsible for removing RNA primers and replacing them with DNA?
A
Primase.
B
DNA Helicase.
C
DNA Polymerase III.
D
DNA Polymerase I.
Verified step by step guidance1
Understand the role of RNA primers in DNA replication: RNA primers are short sequences of RNA nucleotides that provide a starting point for DNA synthesis.
Identify the function of each enzyme listed: Primase synthesizes RNA primers; DNA Helicase unwinds the DNA double helix; DNA Polymerase III extends the DNA strand by adding nucleotides.
Recognize the specific function of DNA Polymerase I: It removes RNA primers and fills in the gaps with DNA nucleotides.
Consider the process of DNA replication: After the RNA primers are laid down by Primase, DNA Polymerase III extends the DNA strand, but the RNA primers need to be replaced with DNA.
Conclude that DNA Polymerase I is the enzyme responsible for removing RNA primers and replacing them with DNA, completing the DNA strand synthesis.
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